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KMID : 0380619930250030197
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Korean Journal of Food Science and Technology
1993 Volume.25 No. 3 p.197 ~ p.203
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Screening of Complement - System Activating Polysaccharide from Edible Plants and Its Action Mode
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Abstract
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Screenings were performed on edible plants to examine their complement-system activating ability (anti-complementary activity) by hemolytic complement assay (TCH_(50). Among 38 kinds of plant extracts, 5 kinds showed relatively strong anti-complementary activity which decreased TCH_(50), more than 60% comparison with control and the order of activity was Zingiber officinale$gt;Colocasia antiquorum$gt;Capsella bursapastaris$gt;Ginkgo biloba$gt;Alium monanthum in 1000 μg/ml. The anti-complementary activity of ZR-1 prepared from the root of Zingiber officinale which was showed the most potent activity, did not change by pronase treatment, but decreased greatly by periodate oxidation. These results indicate that not protein moiety but carbohydrate moiety in ZR-1 fraction may also contribute to the anti-complementary activity. Also, the anti-complementary activity of ZR-1 was reduced partially in the absence of the Ca^(2+) ion. When crossed immunoelectrophoresis using anti-human C3 serum was carried out after incubation of normal human serum with the ZR-1 in Ca^(2+) free condition, a cleavage of C3 precipitin line was observed. Furthermore this polysaccharide fraction considerably inhibited ACH_(50). These results also indicate that the mode of complement activation by polysaccharide from Zingiber officinale is via not only the classical pathway but also the alternative pathway.
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